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Literature summary extracted from

  • Vigetti, D.; Clerici, M.; Deleonibus, S.; Karousou, E.; Viola, M.; Moretto, P.; Heldin, P.; Hascall, V.C.; De Luca, G.; Passi, A.
    Hyaluronan synthesis is inhibited by adenosine monophosphate-activated protein kinase through the regulation of HAS2 activity in human aortic smooth muscle cells (2011), J. Biol. Chem., 286, 7917-7924.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.212 expression of wild-type FLAG-tagged human HAS1, HAS2, or HAS3, and of HAS T110A mutant enzyme in COS-7 cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.212 T110A site-directed mutagenesis of the phosphorylation site residue, the mutant is not inhibited by AMP-activated protein kinase Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.212 2-deoxyglucose
-
 Homo sapiens
2.4.1.212 5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside modulates aortic smooth muscle cell motility and adhesive properties through AMP-activated protein kinase, AMPK Homo sapiens
2.4.1.212 metformin modulates aortic smooth muscle cell motility and adhesive properties through AMP-activated protein kinase, AMPK Homo sapiens
2.4.1.212 additional information AMP-activated protein kinase, AMPK, phosphorylates HAS2 at Thr110, which inhibits its enzymatic activity, and is itself inhibit by Compound C. Isoenzymes HAS1 and HAS3 are not modified by the kinase Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.212 membrane
-
 Homo sapiens 16020
-
2.4.1.212 microsome
-
 Homo sapiens
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.212 Mg2+ required Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.212 Homo sapiens Q92819
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.1.212 phosphoprotein AMP-activated protein kinase, AMPK, phosphorylates HAS2 at Thr110, which inhibits its enzymatic activity. Isoenzymes HAS1 and HAS3 are not modified by the kinase Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.212 aortic smooth muscle cell
-
 Homo sapiens
-

Synonyms

EC Number Synonyms Comment Organism
2.4.1.212 Has2
-
 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.212 7.4
-
 assay at Homo sapiens

General Information

EC Number General Information Comment Organism
2.4.1.212 malfunction reduction of HA due to decreased HAS activity, caused by phosphorylation at Thr110 through AMP-activated protein kinase, decreases the ability of aortic smooth muscle cells to proliferate, migrate, and recruit immune cells, thereby reducing the pro-atherosclerotic AoSMC phenotype. AMP-activated protein kinase can block the pro-atherosclerotic signals driven by HA by interaction with its receptors Homo sapiens
2.4.1.212 physiological function hyaluronan synthesis is inhibited by adenosine monophosphate-activated protein kinase through the regulation of HAS2 activity in human aortic smooth muscle cells Homo sapiens